PoplarV1, Main, Exploration, bibRecord, 000B33

Analysis of condensed tannins in Populus spp. using reversed phase UPLC-PDA-(-)esi-MS following thiolytic depolymerisation.

Identifieur interne : 000B33 ( Main/Exploration ); précédent : 000B32; suivant : 000B34

Analysis of condensed tannins in Populus spp. using reversed phase UPLC-PDA-(-)esi-MS following thiolytic depolymerisation.

Auteurs : Kennedy F. Rubert-Nason [États-Unis] ; Richard L. Lindroth [États-Unis]

Source :

Phytochemical analysis : PCA [ 1099-1565 ] ; 2019.

RBID : pubmed:30548354

Descripteurs français

KwdFr :
- Calibrage (MeSH), Chromatographie en phase liquide à haute performance (méthodes), Feuilles de plante (composition chimique), Gènes de plante (MeSH), Génotype (MeSH), Hydroxylation (MeSH), Limite de détection (MeSH), Normes de référence (MeSH), Polymérisation (MeSH), Populus (composition chimique), Populus (génétique), Spectrométrie de masse ESI (méthodes), Spectrophotométrie UV (méthodes), Stéréoisomérie (MeSH), Tanins (analyse), Tanins (normes), Thiols (composition chimique).
MESH :
- analyse : Tanins.
- composition chimique : Feuilles de plante, Populus, Thiols.
- génétique : Populus.
- méthodes : Chromatographie en phase liquide à haute performance, Spectrométrie de masse ESI, Spectrophotométrie UV.
- normes : Tanins.
- Calibrage, Gènes de plante, Génotype, Hydroxylation, Limite de détection, Normes de référence, Polymérisation, Stéréoisomérie.

English descriptors

KwdEn :
- Calibration (MeSH), Chromatography, High Pressure Liquid (methods), Genes, Plant (MeSH), Genotype (MeSH), Hydroxylation (MeSH), Limit of Detection (MeSH), Plant Leaves (chemistry), Polymerization (MeSH), Populus (chemistry), Populus (genetics), Reference Standards (MeSH), Spectrometry, Mass, Electrospray Ionization (methods), Spectrophotometry, Ultraviolet (methods), Stereoisomerism (MeSH), Sulfhydryl Compounds (chemistry), Tannins (analysis), Tannins (standards).
MESH :
- chemical , analysis : Tannins.
- chemical , chemistry : Sulfhydryl Compounds.
- chemistry : Plant Leaves, Populus.
- genetics : Populus.
- methods : Chromatography, High Pressure Liquid, Spectrometry, Mass, Electrospray Ionization, Spectrophotometry, Ultraviolet.
- chemical , standards : Tannins.
- Calibration, Genes, Plant, Genotype, Hydroxylation, Limit of Detection, Polymerization, Reference Standards, Stereoisomerism.

Abstract

INTRODUCTION

Condensed tannins (CTs) are proanthocyanidin heteropolymers that are widely distributed among plants. Their biochemical properties are determined by molecular structure (e.g. polymer size, hydroxylation, stereochemistry). In Populus, genetically and environmentally-determined CT concentrations have been related to ecological effects, while the potential role of CT molecular structure has received little attention.

OBJECTIVE

Evaluate CT polymerisation, major constituent monomers, stereochemistry and overall content in Populus tremuloides foliage using ultra-high-performance liquid chromatography with photodiode array and mass spectrometry (UPLC-PDA-(-)esi-MS) detection following thiolytic depolymerisation of the CTs.

METHODOLOGY

CTs were extracted from dried foliage of six P. tremuloides genotypes into methanol and thiolytically depolymerised into constituent monomers. Calibration standards were prepared by thiolysis of CT mixtures isolated from P. tremuloides foliage on Sephadex LH-20, followed by preparative high-performance liquid chromatography (HPLC).

RESULTS

Populus tremuloides CTs contained predominantly repeating subunits of three putative stereoisomers each of catechin and gallocatechin. Linear calibrations for standards of these subunits and their thioethers (purities 44-87%, UPLC-(-)esi-MS) were generally stable over the course of 10 months. CT polymer size, hydroxylation, stereochemistry and concentrations differed among genotypes.

CONCLUSION

This thiolysis-UPLC-PDA-(-)esiMS method was optimised for analysis of CT polymer size, hydroxylation, stereochemistry, and total concentration in Populus foliage. It revealed significant variation in each of these properties among P. tremuloides genotypes, and will facilitate evaluation of how environmental factors affect CT molecular structures.

DOI: 10.1002/pca.2810
PubMed: 30548354

Affiliations:

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Le document en format XML

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<author><name sortKey="Rubert Nason, Kennedy F" sort="Rubert Nason, Kennedy F" uniqKey="Rubert Nason K" first="Kennedy F" last="Rubert-Nason">Kennedy F. Rubert-Nason</name>
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<term>Genes, Plant (MeSH)</term>
<term>Genotype (MeSH)</term>
<term>Hydroxylation (MeSH)</term>
<term>Limit of Detection (MeSH)</term>
<term>Plant Leaves (chemistry)</term>
<term>Polymerization (MeSH)</term>
<term>Populus (chemistry)</term>
<term>Populus (genetics)</term>
<term>Reference Standards (MeSH)</term>
<term>Spectrometry, Mass, Electrospray Ionization (methods)</term>
<term>Spectrophotometry, Ultraviolet (methods)</term>
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<term>Tannins (standards)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Calibrage (MeSH)</term>
<term>Chromatographie en phase liquide à haute performance (méthodes)</term>
<term>Feuilles de plante (composition chimique)</term>
<term>Gènes de plante (MeSH)</term>
<term>Génotype (MeSH)</term>
<term>Hydroxylation (MeSH)</term>
<term>Limite de détection (MeSH)</term>
<term>Normes de référence (MeSH)</term>
<term>Polymérisation (MeSH)</term>
<term>Populus (composition chimique)</term>
<term>Populus (génétique)</term>
<term>Spectrométrie de masse ESI (méthodes)</term>
<term>Spectrophotométrie UV (méthodes)</term>
<term>Stéréoisomérie (MeSH)</term>
<term>Tanins (analyse)</term>
<term>Tanins (normes)</term>
<term>Thiols (composition chimique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Tannins</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Sulfhydryl Compounds</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term>
<term>Populus</term>
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<term>Thiols</term>
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<term>Spectrometry, Mass, Electrospray Ionization</term>
<term>Spectrophotometry, Ultraviolet</term>
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<term>Spectrométrie de masse ESI</term>
<term>Spectrophotométrie UV</term>
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<keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Tanins</term>
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<keywords scheme="MESH" type="chemical" qualifier="standards" xml:lang="en"><term>Tannins</term>
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<keywords scheme="MESH" xml:lang="en"><term>Calibration</term>
<term>Genes, Plant</term>
<term>Genotype</term>
<term>Hydroxylation</term>
<term>Limit of Detection</term>
<term>Polymerization</term>
<term>Reference Standards</term>
<term>Stereoisomerism</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Calibrage</term>
<term>Gènes de plante</term>
<term>Génotype</term>
<term>Hydroxylation</term>
<term>Limite de détection</term>
<term>Normes de référence</term>
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<front><div type="abstract" xml:lang="en"><p><b>INTRODUCTION</b>
</p>
<p>Condensed tannins (CTs) are proanthocyanidin heteropolymers that are widely distributed among plants. Their biochemical properties are determined by molecular structure (e.g. polymer size, hydroxylation, stereochemistry). In Populus, genetically and environmentally-determined CT concentrations have been related to ecological effects, while the potential role of CT molecular structure has received little attention.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b>
</p>
<p>Evaluate CT polymerisation, major constituent monomers, stereochemistry and overall content in Populus tremuloides foliage using ultra-high-performance liquid chromatography with photodiode array and mass spectrometry (UPLC-PDA-(-)esi-MS) detection following thiolytic depolymerisation of the CTs.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODOLOGY</b>
</p>
<p>CTs were extracted from dried foliage of six P. tremuloides genotypes into methanol and thiolytically depolymerised into constituent monomers. Calibration standards were prepared by thiolysis of CT mixtures isolated from P. tremuloides foliage on Sephadex LH-20, followed by preparative high-performance liquid chromatography (HPLC).</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Populus tremuloides CTs contained predominantly repeating subunits of three putative stereoisomers each of catechin and gallocatechin. Linear calibrations for standards of these subunits and their thioethers (purities 44-87%, UPLC-(-)esi-MS) were generally stable over the course of 10 months. CT polymer size, hydroxylation, stereochemistry and concentrations differed among genotypes.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>This thiolysis-UPLC-PDA-(-)esiMS method was optimised for analysis of CT polymer size, hydroxylation, stereochemistry, and total concentration in Populus foliage. It revealed significant variation in each of these properties among P. tremuloides genotypes, and will facilitate evaluation of how environmental factors affect CT molecular structures.</p>
</div>
</front>
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<Month>05</Month>
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<Month>May</Month>
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<ArticleTitle>Analysis of condensed tannins in Populus spp. using reversed phase UPLC-PDA-(-)esi-MS following thiolytic depolymerisation.</ArticleTitle>
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<Abstract><AbstractText Label="INTRODUCTION" NlmCategory="BACKGROUND">Condensed tannins (CTs) are proanthocyanidin heteropolymers that are widely distributed among plants. Their biochemical properties are determined by molecular structure (e.g. polymer size, hydroxylation, stereochemistry). In Populus, genetically and environmentally-determined CT concentrations have been related to ecological effects, while the potential role of CT molecular structure has received little attention.</AbstractText>
<AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">Evaluate CT polymerisation, major constituent monomers, stereochemistry and overall content in Populus tremuloides foliage using ultra-high-performance liquid chromatography with photodiode array and mass spectrometry (UPLC-PDA-(-)esi-MS) detection following thiolytic depolymerisation of the CTs.</AbstractText>
<AbstractText Label="METHODOLOGY" NlmCategory="METHODS">CTs were extracted from dried foliage of six P. tremuloides genotypes into methanol and thiolytically depolymerised into constituent monomers. Calibration standards were prepared by thiolysis of CT mixtures isolated from P. tremuloides foliage on Sephadex LH-20, followed by preparative high-performance liquid chromatography (HPLC).</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Populus tremuloides CTs contained predominantly repeating subunits of three putative stereoisomers each of catechin and gallocatechin. Linear calibrations for standards of these subunits and their thioethers (purities 44-87%, UPLC-(-)esi-MS) were generally stable over the course of 10 months. CT polymer size, hydroxylation, stereochemistry and concentrations differed among genotypes.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">This thiolysis-UPLC-PDA-(-)esiMS method was optimised for analysis of CT polymer size, hydroxylation, stereochemistry, and total concentration in Populus foliage. It revealed significant variation in each of these properties among P. tremuloides genotypes, and will facilitate evaluation of how environmental factors affect CT molecular structures.</AbstractText>
<CopyrightInformation>© 2018 John Wiley & Sons, Ltd.</CopyrightInformation>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rubert-Nason</LastName>
<ForeName>Kennedy F</ForeName>
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<AffiliationInfo><Affiliation>Department of Entomology, University of Wisconsin-Madison, Madison, WI, USA.</Affiliation>
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<Agency>National Science Foundation</Agency>
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<Grant><Agency>US Department of Agriculture</Agency>
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<Keyword MajorTopicYN="N">UPLC</Keyword>
<Keyword MajorTopicYN="N">acid-butanol</Keyword>
<Keyword MajorTopicYN="N">aspen</Keyword>
<Keyword MajorTopicYN="N">catechin</Keyword>
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Analysis of condensed tannins in Populus spp. using reversed phase UPLC-PDA-(-)esi-MS following thiolytic depolymerisation.

Analysis of condensed tannins in Populus spp. using reversed phase UPLC-PDA-(-)esi-MS following thiolytic depolymerisation.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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